Büllöz pemfigoidli hastalarda serum ve lezyonel miRNA-1291 ekspresyonlarının karşılaştırılması

Purpose: Bullous pemphigoid (BP) is an autoimmune disease increasing by age. miRNA-1291 a microRNA that plays major role in many immune-mediated cutaneous diseases. We aimed to investigate the of BP comparing its expression levels serum and lesional skin correlation with other biomarkers (CCL17, anti-BP180, anti-BP230).
 Materials methods: Twenty three patients 23 healthy control were included study. The miRNeasy Mini Kits was used for RNA isolation from biopsy samples. Qiagen-miRCURY LNA RT Kit conversion cDNA. performed Real-Time PCR original primer SYBR Green master mix. Serum CCL17, anti-BP230 measured use ELISA. SPSS 25.0 (IBM Statistics 25 software) statistical evaluation.
 Results: samples 7.02 4.77-fold significantly upregulated than group respectively (p=0.001, p=0.007). anti-BP-180, anti-BP-230 CCL17 higher compared (p=0.0049, p=0.0071, p=0.001). Anti-BP180, anti-BP230, correlated positively patients. Additionally, well expression.
 Conclusion: increase tissue non-lesional suggests may play pathogenesis BP. As correlates biomarkers, it serve as important biomarker